Participant 10: UR1
Inserm U620, Université de Rennes 1, France
Participant leader: Prof. André Guillouzo
Relevant experience and laboratory resources
The INSERM laboratories working in the field of the liver are formed by two joint research units between INSERM and Université de Rennes 1: the unit 620 headed by Prof A. Guillouzo and the unit 522 headed by Dr Christiane Guguen-Guillouzo, within the University of Rennes 1.
They have a long history in the development of liver cell models of both human and animal origin, their functional characterization and their use for xenobiotic metabolism and toxicity studies. The INSERM laboratories are fully equipped with cellular and molecular technologies including DNA chips, RT-PCR, microspectrometry, HPLC, and flux cytometry. They have experience in the fields of gene expression and regulation, gene transfection and RNA silencing.
INSERM units are funded by the government (Ministry of research, INSERM) and caritative foundations as well as by EC (Toxdrop project focused on nano-drop cell chip system).
Principal staff involved:
Andre Guillouzo (M), Université de Rennes 1, is a Professor of Toxicology and is board certified by the French Toxicology Society (EUROTOX). He has published more than 250 peer-reviewed articles and has received several awards and honours including the “French citation laureate” (1981-1998) in 2001. He served as the Editor in Chief of Cell Biology and Toxicology (1997-2002).
Dr. Christiane Guguen-Guillouzo (F), INSERM, Director of Research, and head of the INSERM unit 522, has a long expertise in hepatocyte cultures and has been involved in the development of the human hepatoma HepaRG cell line
Dr. Caroline Aninat (F), Université de Rennes 1, is an Assistant-Professor of Toxicology; she has expertise in liver cell cultures, analysis of phase 1 and phase 2 enzymes, DNA repair systems and cytotoxicity markers.
Denise Glaise (F), INSERM, is an engineer having a long expertise in liver cell cultures including HepaRG cells and differentiation.
A post-doc is to be appointed
Role of the participant in the project and main tasks
The participant is responsible for HepaRG cell cultures and their analysis of their functional properties (WP2).