Participant 17: U.A
Université d’Artois, Faculté Jean Perrin, 62307 Lens, France
Participant leader: Dr. Romeo Cecchelli
Relevant experience and laboratory resources:
Romeo Cecchelli and his team have 20 years experience in blood-brain barrier research. They were the first to set up a coculture model of bovine brain capillary endothelial cells and rat glial cells that mimics the in vivo conditions. The good in vitro – in vivo correlation supports the use of this coculture system as relevant model for making predictions of drug transport to the brain. This model does not require sophisticated methods and equipment. It is already used in different academic laboratories and pharmaceutical companies. They are qualified to produce a state of art level in vitro culture of BBB. They were the first to demonstrate the transcellular transport of blood-borne molecules using the caveolae pathway that bypass the degradative lysosomial compartment. Taking into account their background in endothelial cell culture and coculture, they will provide a new in vitro 4 days BBB model in 24 well-plates which can be used for primary screening (HTS). They will focus their work on the role of the BBB in neurotoxicity using neuronal and glial cell cultures. The group has notable experience in other European Union projects including FP6 ACUTE-TOX (WP5) and European Commission contract n°CCR.IHCP. C431931.XO: 2006-2007. Contractor: Romeo Cecchelli “Evaluation of the performance of five selected in vitro blood-brain barrier for predicting absorption/uptake the brain”.
Principal staff involved:
Prof. Cecchelli Romeo – Vice President of the university d’Artois in charge of research and Director of BBB Laboratory.
Prof. Dehouck Marie-Pierre – principal investigator, experience in cell culture.
Responsibility: in charge of the development of the new in vitro model
Dehouck Lucie (F) – in vitro models, preparation of neuronal and glial cell culture. Immunohistochemistry and cytochemistry.
Sevin Emmanuel (M) – Responsible of the automatisation of the model.
Role of the participant in the project and main tasks:
The participant will contribute in WP2.3 and WP3.